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Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
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Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
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Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
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Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
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MathWorks Inc software program developed in-house using a spline-based semi-automated segmentation algorithm
Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
Software Program Developed In House Using A Spline Based Semi Automated Segmentation Algorithm, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
Software Program, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MathWorks Inc custom matlab-based program version 2016r
Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
Custom Matlab Based Program Version 2016r, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MathWorks Inc spline-based semi-automated segmentation algorithm
Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
Spline Based Semi Automated Segmentation Algorithm, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program <t>CSDplotter.</t> Euth, euthanasia.
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Image Search Results


Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program CSDplotter. Euth, euthanasia.

Journal: The Journal of Neuroscience

Article Title: Prolonged Deficits in Parvalbumin Neuron Stimulation-Evoked Network Activity Despite Recovery of Dendritic Structure and Excitability in the Somatosensory Cortex following Global Ischemia in Mice

doi: 10.1523/JNEUROSCI.1775-14.2014

Figure Lengend Snippet: Sixteen-channel optrode laminar recording of PV-ChR2-mediated local field potential signals and CSD analysis in the cortex. A, A 16-channel optrode (OA16; NeuroNexus Technologies) with an optical fiber attached at ∼50 μm above the first recording site was placed into the somatosensory cortex of AAV.DIO.ChR2-injected PV-Cre mice for local field potential recording and ChR2 stimulation. The contralateral forepaw was electrically stimulated to induce sensory cortical responses for comparison. B, Left, A diagram shows the distribution of each recording site of the optrode in the somatosensory cortex. Right, 10 ms, 5 mW of blue laser PV-ChR2 stimulation-evoked LFP signals corresponding to laminar layers before (black) and after being killed (red). C, CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program CSDplotter. Euth, euthanasia.

Article Snippet: C , CSD analysis is performed with cubic spline iCSD method based on 16-channel LFP signals using the MATLAB-based program CSDplotter.

Techniques: Injection, Comparison